Product Technology & Features

The EXO-NET® solution – Immuno-Magnetic Bead Based Capture System

Despite the explosion in exosome research and their potential in disease (especially cancer) diagnostics, exosome-associated biomarkers have not yet been translated to meet the rapid developments in molecular medicine for commercial liquid biopsy applications. This is primarily due to the limitations of current exosome capture and isolation methods, which are time consuming, operator dependent and not scalable. These limitations are overcome by using INOVIQ’s EXO-NET® technology.

Comparison of the key features in commonly used EV enrichment techniques1

EV enrichment techniques

Time

Cost

Scalability

Recovery

Specificity

Polyethylene glycol precipitation

+++

++++

++++

++++

+

Size exclusion chromatography

+

+

+

+

+++

High MW centrifugal filters

++++

+++

++++

+++

++

Differential ultracentrifugation

+

++

+

+

++

Tangential flow filtration

+++

++

++++

+++

+++

Affinity chromatography

++

+

++

++

++++

Immuno-magnetic bead capture*

++++

+++

+++++

+++

++++

*EXO-NET® is an immuno-magnetic bead capture system

1 Extracellular Vesicles and Their Emerging Roles as Cellular Messengers in Endocrinology: An Endocrine Society Scientific St atement
Carlos Salomon, Saumya Das, Uta Erdbrügger, Raghu Kalluri, Sai Kiang Lim, Jerrold M. Olefsky, Gregory E. Rice, Susmita Sahoo, W. Andy Tao, Pieter Vader, Qun Wang, and Alissa
M. Weaver. Endocrine Reviews, 2022, 43, 441–468

INOVIQ’s  EXO-NET® allows for improved purity and yield, fast and efficient capture and isolation of extracellular vesicles from any liquid biopsy sample. The technology is highly scalable and is compatible with existing automated testing systems making it ideal for high volume research and commercial laboratory applications used in the analysis of proteins, lipids and nucleic acids.

Product features
EXO-NET® Pan -Exosome Capture features
UNIQUE COMPOSITION Magnetic bead-based immunoaffinity EV capture technology that is comprised of a multi-antibody matrix around magnetic nanobeads to enhance EV capture
CUSTOMISABLE Capture of exosome subtypes using specific antibodies
PURITY Reduced co-isolation of contaminants
FAST 15 minutes EV capture
SCALABILITY Suitable for automation and high-throughput screening
DOWNSTREAM COMPATIBILITY Compatible for use with most downstream application
SAMPLE VERSATILITY Optimal solution for very low volume and rare samples from plasma, urine, saliva and cell culture
DISCOVERY EXO-NET® products in development can be used to isolated specific EVs for in use in target disease indications